Coding
Part:BBa_I725016:Design
Designed by: Erin Dul Group: iGEM07_Alberta (2007-06-18)
Thiolase
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This sequence has been optimized for synthesis in E. Coli and has had its restriction sites removed. It has a C terminal His tag. It has had its stop codon mutated to TAA.
Source
C. acetobutylicum genom. CAC_2873