Coding

Part:BBa_I725016:Design

Designed by: Erin Dul   Group: iGEM07_Alberta   (2007-06-18)


Thiolase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This sequence has been optimized for synthesis in E. Coli and has had its restriction sites removed. It has a C terminal His tag. It has had its stop codon mutated to TAA.


Source

C. acetobutylicum genom. CAC_2873

References